Figure 1a: Experimental Agar Ampicillin plate spread with manipulated E. coli bacterial cells containing plasmid. Growth of bacteria developed after plate was stored in the incubator overnight. Droplets on plate indicate condensation but while tiny ‘dot’ is an individual bacterial colony. Plate is labeled in black sharpie to indicate contents, initials and date.
Figure 1b: Control Agar Ampicillin plate spread with E. coli bacterial cells that do NOT contain any plasmid. Plate was stored in the incubator overnight. Droplets on plate indicate condensation. Plate is labeled in black sharpie to indicate contents, initials and date.
Figure 1c: ‘Fun’ Agar plate containing NO Ampicillin swabbed with mucus sample. Growth of bacteria developed after plate was stored in the incubator overnight. Droplets on plate indicate condensation but while tiny ‘dot’ is an individual bacterial colony. Plate is labeled in sharpie to indicate contents, initials and date.
Figure 1d: Experimental Agar Ampicillin plate spread with manipulated E. coli bacterial cells containing plasmid. Each tiny ‘dot’ is an individual bacterial colony. The additional growth of the E. coli bacteria developed after plate was stored in the incubator for an additional 16-24 hours. Plate is labeled in black sharpie to indicate contents, initials and date.
Figure 2: Manipulated bacterial cells after allowed incubation for 16-24 hours. Cloudy nature of solution indicates significant bacterial growth. Purple color of culture represents successful expression of protein by the manipulated bacterial cells. Flask is labeled with tape and sharpie to indicate contents, (Bl21, PGEM-gbr22) initials and date.
Figure 3: Bacterial cells after allowed ten minutes time in 4 degree Celsius benchtop centrifuge for ten minutes. Cells condensed into purple-colored pellet at bottom of tube while rest of spent LB media was properly disposed of. Cell pellet weigh measured approximately 54 grams. Purple color of cells represents successful expression of protein by the manipulated bacterial cells. Flask is labeled with tape and sharpie to indicate contents, (Bl21, PGEM-gbr22) initials and date.
Figure 4: Elution 1 after flow through Ni-NTA column with 250mM imidazole. The diluted purple color of the solution represents the presence of the PGEM-gbr22 protein.
Figure 5: Elution 2 after second flow through Ni-NTA column with 250mM imidazole. The clear color of the solution represents little to no presence of the PGEM-gbr22 protein.
Figure 6: Gel prior to being dried. Purple color of bands is due to color of stain. Far left band represents the “Ladder,” then proceeding left to right each subsequent band represent my Samples 1, 2, 3, 4, 5, 6, and the final two bands represent Ariana’s Sample 5 and 6.
Figure 7: Gel following being dried. Purple color of bands is due to color of stain. Gel is covered tightly with layer of clear Saran Wrap. Far left band represents the “Ladder,” then proceeding left to right each subsequent band represent my Samples 1, 2, 3, 4, 5, 6, and the final two bands represent Ariana’s Sample 5 and 6.
Introduction:
Materials & Methods:
Results:
Discussion:
Conclusions:
References: